Dissecting the mechanism of oxygen trafficking in a metalloenzyme.
نویسندگان
چکیده
A key question in the biological activation of oxygen is how the protein matrix regulates the delivery of oxygen to its site of activation. We are using Escherichia coli copper amine oxidase as a model system to investigate the roles played by both local active site residues as well as long range interactions in this process. We have generated active site mutants, as well as mutants in the putative oxygen delivery channel and characterised their affects on enzyme structure and catalysis.
منابع مشابه
Computational Model of Reaction Mechanism of Alkyl Peroxy Radicals with Organic Compounds in the Presence and Absence of Oxygen
On the basis of experimental data a kinetic model for the heterogeneous interaction between alkylperoxyradicals and organic compounds in Langmuir- Hinshelwood approach at room temperature has been offered.The effect of oxygen on the kinetics of process in the presence, [O2]o = 1 x 1011 – 1.6 x 1012 molecules.cm-2, and absence of oxygen has been analyzed. Over time the chain degenerate branching...
متن کاملInternational Human Trafficking: Critical Appraisal of Strength and Weaknesses of Nigeria’s Legal Frameworks
Nigeria has been facing the immense challenge of international trafficking in persons for sexual exploitation and forced labor into countries in Europe, Asia and Africa. Being an international criminal activity driven by high profits, Nigeria's adoption of a regulatory policy is expressed in the series of legal provisions to deal with the army of traffickers. In desperation, within a space of 1...
متن کاملA tri state mechanism for oxygen release in fish hemoglobin: Using Barbus sharpeyi as a model
Hemoglobin is a porphyrin containing protein with an a2b2 tetrameric structure and like other porphyrin compounds shows spectral behavior of species specific characteristics. Researchers tend to relate bands in the hemoglobin spectra to certain structural and/or functional features. Given the fact that hemoglobin is the main oxygen carrier in animals functioning through the Oxy«Deoxy equilibriu...
متن کاملA Meta-Analysis of the Factors Affecting Drug Trafficking and Strategies to Deal with it during 2006 to 2017 in Tehran *
Objective: The present study aimed to conduct a meta-analysis of factors affecting drug trafficking and strategies to deal with it from 2006 to 2017 in Tehran. Method: The research method was meta-analysis. A total of 18 studies in the field of drug trafficking were identified through searching on the Internet in domestic databases and in organizations and universities in Tehran, and finally, d...
متن کاملRab11 in Disease Progression
Membrane/ protein trafficking in the secretory/ biosynthetic and endocytic pathways is mediated by vesicles. Vesicle trafficking in eukaryotes is regulated by a class of small monomeric GTPases the Rab protein family. Rab proteins represent the largest branch of the Ras superfamily GTPases, and have been concerned in a variety of intracellular vesicle trafficking and different intracellular sig...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Faraday discussions
دوره 148 شماره
صفحات -
تاریخ انتشار 2011